By Arie Altman

This paintings integrates simple biotechnological methodologies with updated agricultural practices, supplying options to express agricultural wishes and difficulties from plant and crop yield to animal husbandry. It offers and evaluates the restrictions of classical methodologies and the possibility of novel and emergent agriculturally comparable biotechnologies.

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2. Axillary Bud Proliferation Axillary bud proliferation in vitro is usually considered a convenient route for micropropagation. Because it does not include a callus stage, it is considered “safer” for the preservation of clonal characteristics (see foregoing). Bud meristems already exist in the axils of leaves, but because of apical control, they normally do not develop in planta until the stem elongates and grows. Thus, the short stem tip contains many axillary buds at different stages of development, condensed in a small explant.

Production of pathogenfree plants and of genetically engineered plants) are dealt with separately in other chapters of this book. II. WHY IN VITRO CLONAL PROPAGATION? A. Generative (Sexual) and Vegetative Propagation (Cloning) Plant reproduction by seeds (sexual propagation) is routinely used, is usually inexpensive, and is easy for most field crops and vegetables. This is possible in homozygous plants and in annual plants for which pure seeds can be produced by backcrossing, in a relatively short time.

Ernst & Young, Biotechnology 1996 (North America), Biobusiness Annu. , 1996. Hoechst AG, Development of Crop Protection in World Market. 1995, 78 pp. Study predicts biotechnology to play a significant role, Seed Trade News, pp. 8–9, (1994). Curtis, Delivering bio control in the tropics, Nature Biotechnol. 14:265 (1996). Carver, Transgenic on trial, Scrip Mag. Nov: 51–53 (1996). Biotechnology and genetics—breaking nature’s limits, Economist, Feb 25:3–18 (1995). Smith Barney Harris, A guide of who is who and what is what in recombinant DNA technology, Bio Venture Views, 4PHAM&10.

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